| Analysis Note | Package sizes are based on DEA units |
| Application | High specific activity grade recommended for antibody and protein conjugation. |
| Biochem/physiol Actions | The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids. |
| Unit Definition | One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units) |
| Physical form | Solution in 50% glycerol containing 5 mM Tris, 5 mM MgCl2 and 0.1 mM ZnCl2, pH 7.0 |
| | Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5'-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.. |
| Physical properties | Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity. |
| IUBMB